Confocal Microscopy Lab Essay -- Ovarian Ring Canal Medical Technology

Confocal Microscopy Lab Confocal microscopy uses a laser that produces excitation light. This laser light reflects off of a dichroic mirror and then the laser light hits two mirrors that are mounted on motors. The mirrors then scan the laser light across the sample. Dye that is found in the sample then fluoresces (Weeks, 2003). Filamin was labeled with a red fluorescent label rhodamine (TRITC) and actin was labeled with the green fluorescent label fluoroscein (FITC) which was conjugated to the actin-binding fungal toxin phalloidin. The emitted light from the dyes passes back through the mirrors and passes through the dichroic mirror and is focuses into a pinhole. With confocal microscopy, a complete image of the sample is never seen. Only one point of the sample can be observed at a time. The amount of light that passes back through is detected by the microscope (Ladic, 1995). The intensity of the red light seen is proportional to the amount of filamin present and the intensity of the green ligh t seen is proportional to the amount of actin present in the sample of Drosophila melanogaster ovaries. Confocal microscopy is able to produce images that are very free from interference. The confocal pinhole allows the microscope to reject out of focus fluorescent light (Weeks, 2003). This means that the image comes from a thin section of the ovary sample. Many thin sections will be scanned through the sample; this allows a clean three dimensional image to be made. A confocal microscope has a few advantages over regular optical microscopes. Confocal microscopes have controllable depth of field, the elimination of image degrading information that is out of focus, and the ability to collect series of data from s... .../ladic/overview.html. Accessed 6 December 2004. Robinson, D.N., K. Cant and L. Cooley. 1994. "Morphogenesis of Drosophila ovarian ring canals." Development. 120, 2015-2025. Robinson, D.N, T.A. Smith-Leiker, N.S. Sokol, A.M. Hudson and L. Cooley. 1997. â€Å"Formation of the Drosophila ovarian ring canal inner rim depends on cheerio.† Genetics. 145, 1063-1072 Shilling, Kristen (David S. Richard). â€Å"Ovarian nurse cell ring canal formation in wild- type and insulin signaling mutant female Drosophila melanogaster.† Tilney, L.G., M.S. Tilney, and G.M. Guild. 1996. â€Å"Formation of actin filament bundles in the ring canals of developing Drosophila follicles.† The Journal of Cell Biology. 133, 61-74. Weeks, Eric. 2003. â€Å"How does a confocal microscope work?† Physics Department, Emory University. http://www.physics.emory.edu/~weeks/confocal/. Accessed 3 December 2004 Confocal Microscopy Lab Essay -- Ovarian Ring Canal Medical Technology Confocal Microscopy Lab Confocal microscopy uses a laser that produces excitation light. This laser light reflects off of a dichroic mirror and then the laser light hits two mirrors that are mounted on motors. The mirrors then scan the laser light across the sample. Dye that is found in the sample then fluoresces (Weeks, 2003). Filamin was labeled with a red fluorescent label rhodamine (TRITC) and actin was labeled with the green fluorescent label fluoroscein (FITC) which was conjugated to the actin-binding fungal toxin phalloidin. The emitted light from the dyes passes back through the mirrors and passes through the dichroic mirror and is focuses into a pinhole. With confocal microscopy, a complete image of the sample is never seen. Only one point of the sample can be observed at a time. The amount of light that passes back through is detected by the microscope (Ladic, 1995). The intensity of the red light seen is proportional to the amount of filamin present and the intensity of the green ligh t seen is proportional to the amount of actin present in the sample of Drosophila melanogaster ovaries. Confocal microscopy is able to produce images that are very free from interference. The confocal pinhole allows the microscope to reject out of focus fluorescent light (Weeks, 2003). This means that the image comes from a thin section of the ovary sample. Many thin sections will be scanned through the sample; this allows a clean three dimensional image to be made. A confocal microscope has a few advantages over regular optical microscopes. Confocal microscopes have controllable depth of field, the elimination of image degrading information that is out of focus, and the ability to collect series of data from s... .../ladic/overview.html. Accessed 6 December 2004. Robinson, D.N., K. Cant and L. Cooley. 1994. "Morphogenesis of Drosophila ovarian ring canals." Development. 120, 2015-2025. Robinson, D.N, T.A. Smith-Leiker, N.S. Sokol, A.M. Hudson and L. Cooley. 1997. â€Å"Formation of the Drosophila ovarian ring canal inner rim depends on cheerio.† Genetics. 145, 1063-1072 Shilling, Kristen (David S. Richard). â€Å"Ovarian nurse cell ring canal formation in wild- type and insulin signaling mutant female Drosophila melanogaster.† Tilney, L.G., M.S. Tilney, and G.M. Guild. 1996. â€Å"Formation of actin filament bundles in the ring canals of developing Drosophila follicles.† The Journal of Cell Biology. 133, 61-74. Weeks, Eric. 2003. â€Å"How does a confocal microscope work?† Physics Department, Emory University. http://www.physics.emory.edu/~weeks/confocal/. Accessed 3 December 2004

Civils rights and liberties

Explain why conceptions civil rights or liberties (choose only one) which are supposed to be granted to all under the constitution, changed so greatly In the second half of the twentieth century. Make sure to discuss the court's role in this shift. A civil right Is a right or privilege that represents protections by government power or things government must secure on behalf of its citizens. Examples of civil rights are freedom of speech, press, and assembly; the right to vote; freedom from Involuntary servitude; and the right to equality In public places.Discrimination ccurs when the civil rights of an individual are denied or interfered with because of their membership In a particular group or class. Various Jurisdictions have enacted statues to prevent discrimination based on a person's race, sex, religion, age, previous condition of servitude, physical limitation, national origin, and sexual orientation. In American history, there are so many clvll rights movements in the second half of the twentieth century and Congress enacted numerous civil rights statues. Many of these are still in force today and protect Individuals from discrimination and from the deprivation of their civil rights.If we want to talk about civil rights of America, we cannot skip a famous person, Martin Luther King. He is a great leader of human rights In American history. He makes himself reasonable to help people of Birmingham fighting against racial discrimination. For example, King says, â€Å"l am In Birmingham because injustice Is here. † In 1963, he gathered a lot of people in Washington DC and gave his famous â€Å"l have a dream† speech. He inspired his followers; passions and triggered their eagerness to listen to his solutions to unjust situations. Declared in US Constitution, every American is guaranteed civil rights.I think it was not until 1791, that the Bill of Rights was appended to the constitution, which helped clarify these rights to citizens. The famous Dawood stated that rights were eventually applied against actions of the state governments in a series of cases decide by the Supreme Court. Actually, In previous years, Supreme Court had little say in decisions being made by government. As time went on, the Supreme Court took on more responsibility and started making additional decisions, which In time helped minorities gain their civil rights. In 1954, the landmark trial Brown vs. theBoard of Educauon of Topeka Kansas ruled that segregation In public education was unfair. â€Å"We conclude unanimously that in the field of public education the doctrine of Separate but equal' has no place. Separate educational facilities are inherently unequal. † Many African-Americans waited to hear this quote from Chief Justice Earl Warren after many years of fighting for better educational opportunities by means of school desegregation. That decision changed American history and culture forever. Till this day, it effects our school system s and without that decision changed, who knows what could have and what could have not happened. n civils rights and liberties By ryanheinl to be granted to all under the constitution, changed so greatly in the second half of A civil right is a right or privilege that represents protections by government involuntary servitude; and the right to equality in public places. Discrimination their membership in a particular group or class. Various Jurisdictions have enacted orientation. In American history, there are so many civil rights movements in the statues. Many of these are still in force today and protect individuals from Martin Luther King. He is a great leader of human rights in American history.He discrimination. For example, King says, â€Å"l am in Birmingham because injustice is here. † In 1963, he gathered a lot of people in Washington D. C and gave his famous â€Å"l decide by the Supreme Court. Actually, in previous years, Supreme Court had little took on more resp onsibility and started making additional decisions, which in time Board of Education of Topeka Kansas ruled that segregation in public education was of ‘separate but equal' has no place.

The Seleucids and Their Dynasty

The Seleucids were the rulers of the eastern part of Alexander the Greats empire from June 312 to 64 B.C. They were Hellenistic Greek kings in Asia. When Alexander the Great died, his empire was carved up. His first generation successors were known as the diadochi. [See map of the Kingdoms of the Diadochi.] Ptolemy took the Egyptian part, Antigonus took the area in Europe, including Macedonia, and Seleucus took the eastern part, Asia, which he ruled until 281. The Seleucids were the members of the dynasty that ruled Phoenicia, Asia Minor, northern Syria and Mesopotamia. Jona Lendering names the modern states that comprise this area as: Afghanistan,Iran,Iraq,Syria,Lebanon,parts of Turkey, Armenia, Turkmenistan, Uzbekistan, and Tajikistan. The followers of the eponymous Seleucus I were known as the Seleucids or the Seleucid Dynasty. Their actual names included Seleucus, Antiochus, Diodotus, Demetrius, Philip, Cleopatra, Tigranes, and Alexander. Although the Seleucids lost parts of the empire over time, including Transoxania, lost to the Parthians in about 280, and Bactria (Afghanistan) around 140-130 B.C., to the nomadic Yuezhi (possibly the Tocahrians) [E. Knoblochs Beyond the Oxus: Archaeology, Art and Architecture of Central Asia (1972)], they held on to parts. It was only in 64 B.C. that the era of Seleucid rule ended when the Roman leader Pompey annexed Syria and Lebanon.